rabbit polyclonal anti ttp  (Abcam)

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Lung fibrosis is induced in ADAR2 overexpressing mice via HuR-induced CTGF signaling

doi: 10.1096/fj.202101511R

Figure Lengend Snippet: List of Primary antibodies used in this study

Article Snippet: Rabbit polyclonal anti-TTP , Abcam, Cambridge, MA , ab83579.

Techniques: Transduction

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Lung fibrosis is induced in ADAR2 overexpressing mice via HuR-induced CTGF signaling

doi: 10.1096/fj.202101511R

Figure Lengend Snippet: List of Primary antibodies used in this study

Article Snippet: TABLE 1 Primary antibodies Source Identifier Rabbit polyclonal anti-CTGF Abcam, Cambridge, MA ab6992 Rabbit polyclonal anti-ADARB1 Proteintech 22248–1–AP Rabbit polyclonal anti-TTP Abcam, Cambridge, MA ab83579 Rabbit polyclonal anti-HuR Santa Cruz, Dallas, TX Sc–5261 Rabbit β-Actin-HRP Signal transduction #4970 Open in a separate window List of Primary antibodies used in this study.

Techniques: Transduction

Journal: Arthritis Research & Therapy

Article Title: Regulation and localization of endogenous human tristetraprolin

doi: 10.1186/ar778

Figure Lengend Snippet: Saturation curve and specificity of the tristetraprolin (TTP) polyclonal antibody. Results are shown as milliequivalents of soluble fluorescein ± SE and are representative of two separate experiments. (a) Nontransfected and transfected 293 cells were stained with increasing concentrations of the rabbit-derived anti-TTP antibody, or a rabbit IgG control, and then with secondary FITC-conjugated donkey antirabbit IgG. (b) Specificity was demonstrated by introducing the TTP peptide at 10 and 50 times the concentration of the antibody used. All bars derive from the transfected phenotype. (c) Confocal microscopy of cells stained as described for TTP and nuclear staining with propidium iodide. Ab = antibodies; MESF = milliequivalents of soluble fluorescein; SE = standard error of the mean.

Article Snippet: The plate was centrifuged, the supernatant was aspirated, and 3.8 μg per 5 × 10 5 cells of anti-TTP rabbit polyclonal or control antibody (DAKO A/S, Glostrup, Denmark) was added for 45 min more.

Techniques: Transfection, Staining, Derivative Assay, Concentration Assay, Confocal Microscopy